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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Mol Cancer Res. 2011 May 2;9(6):724–732. doi: 10.1158/1541-7786.MCR-10-0436

FIGURE 2. Validation of TNK1 knockdown following siRNA treatment.

FIGURE 2

BxPC3 cells were incubated with one of three different siRNA sequences against TNK1, negative control non-silencing siRNA (NS) or left untreated (UT) and allowed to grow for 48 hours prior to RNA purification or 72 hours prior to protein lysate preparation. A, Silencing of TNK1 by siRNA decreased TNK1 expression by qRT-PCR analysis. All samples were normalized against GAPDH. B, Silencing of TNK1 by siRNA decreased TNK1 protein expression in BxPC3 pancreatic cancer cells as determined by Western blot analysis. Anti-β-tubulin is used as a loading control. Final images were cropped to highlight relevant bands.