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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Mol Cancer Res. 2011 May 2;9(6):724–732. doi: 10.1158/1541-7786.MCR-10-0436

FIGURE 3. Characterization of TNK1 expression in a panel of pancreatic cancer cell lines.

FIGURE 3

A, Whole cell lysates from nine different pancreatic cancer cell lines were separated by SDS-PAGE and analyzed by western blot analysis. Membranes were probed for expression of TNK1, β-actin (loading control), SMAD4, vimentin, and E-cadherin. Final images were cropped to highlight relevant bands. B, Densitometry analysis was completed to compare levels of TNK1 normalized against the loading control. C, Whole-cell lysates were prepared from TNK1 and SMAD4 siRNA-treated Panc-1 cells, which were then resolved by SDS-PAGE and analyzed for TNK1, SMAD4, and β-tubulin expression relative to untreated (UT) and non-silencing (NS) siRNA controls. D, Densitometry analysis was completed to compare protein levels normalized against the loading control.