Figure 5.

Raman spectra and TUNEL staining of SWCNT-PL-PEG-NH₂ and MWCNT-PL-PEG-NH₂ in 2D cell culture in vitro. OVCAR8 ovarian cancer cells treated with LP dispersed SWCNTs and MWCNTs for 3 hrs were subjected to Raman analysis to detect the presence of nanotubes using a handheld portable iRaman spectrometer with a 785 nm laser source. The untreated control well (A) did not show any nanotube peak while SWCNTs (C) and MWCNTs (E) treated cells indeed showed the characteristic G peaks. TUNEL staining was done on irradiated cells in the presence and absence of CNTs to detect apoptosis. Control cells (B) did not show any green fluorescence indicating that the irradiation did not prompt apoptosis. While cells irradiated in the presence of both SWCNT-PL-PEG-NH₂ (D) and MWCNT-PL-PEG-NH₂ (F) showed green fluorescence indicating the presence of apoptosis.