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. 2001 May 15;15(10):1217–1228. doi: 10.1101/gad.884001

Figure 6.

Figure 6

Intracellular localization of GFP-Yak1p. (A) Localization of GFP-Yak1p is regulated by glucose. The yak1Δ (H214) cells harboring the single-copy and GFP-Yak1p-expressing plasmid (pHIS063) grown in SCM-Ura were collected and resuspended in SCM-Ura (+Glc) or SCM-Ura minus glucose (−Glc). After 3 min, fluorescent images were taken. (B) Effect of the glucose analogs on the nuclear localization of GFP-Yak1p. The glucose analogs shown were added to pHIS063/H214 cells precultured in SCM-Ura minus glucose. (Glc) D-glucose; (L-Glc) L-glucose; (6-Deo-Glc) 6-deoxy-D-glucose; (2-Deo-Glc) 2-deoxy-D-glucose. (C) Time course of the translocation of GFP-Yak1p. Images were taken for the time periods indicated after the pHIS063/H214 cells were transferred to SCM-Ura from SCM-Ura minus glucose (+Glc), or SCM-Ura minus glucose from SCM-Ura (−Glc).