Table 1.
Purification of Pop2 peptide kinase
| Fraction
|
Volume (mL)
|
Total protein (μg)
|
Total activity (cpm/min)
|
Specific activity (cpm/min/mg)
|
Enrichment (fold)
|
|---|---|---|---|---|---|
| P11 | 60 | 216,000 | 71,600 | 0.331 | 1 |
| Methyl-HIC | 12 | 13,400 | 408,000 | 30.4 | 92 |
| Bio-Gel HTP (NaCl) | 4.5 | 563 | 335,000 | 595 | 1800 |
| Bio-Gel HTP (Phosphate) | 4.5 | 59.6 | 219,000 | 3670 | 11,000 |
| Bio-Scale CHT2-I | 0.4 | [10]a | 38,200 | [3820]a | [11,500]a |
a
Quantity of total protein in Bio-Scale CHT2-1 peak fraction estimated by Coomassie Brilliant Blue staining on SDS-PAGE.