Figure 4.

Identification of the TEAD protein binding domain in YAP. (A) Each YAP protein used in B is shown, together with the map number of their terminal amino acid and the efficiency with which they bound full-length TEAD–2 protein. The TEAD binding domain (aa 32–139), the 14-3-3 binding domain (Kanai et al. 2000), protein binding domains WW, SH3, and TWL (shaded bars), and the YAP transcriptional activation domain (solid bar) are indicated (Yagi et al. 1999). (B) The indicated full-length GST–YAP protein (YAP), YAP(–TEAD bd), a deletion of amino acids 77 to 96, and YAP protein fragments (A to S) were attached to beads and then incubated with full-length [³⁵S]TEAD-2 protein to determine their binding efficiency (“% TEAD-2 binding”), as described in Figure 2.