Figure 5.

TEAD-dependent transcription requires YAP. Mouse 3T3 embryonic fibroblasts (which express YAP and TEAD-1, -2, -3, and -4 genes) or EL-4 T-lymphocytes (which express neither YAP nor TEAD proteins) were transfected with a mixture of three plasmids: pGT₄Tluc, which expresses firefly luciferase only in the presence of a TEAD transcription factor; an increasing amount of pCI[H–TEAD–2], which expresses mouse H–TEAD–2 to activate transcription of the luciferase gene in pGT₄Tluc; and pRI(βgal), which expresses Escherichia coli β–galactosidase to measure transfection efficiency. The amount of luciferase activity (measured as light units) was normalized to the amount of β–galactosidase activity in each assay. Where indicated (solid symbols), a fixed amount of pSI(FH-mYAP) was included to produce mouse YAP protein to determine whether or not YAP was required for TEAD-dependent transcription. The mean values (±sem) from three independent experiments are given.