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CD spectra of PNA1 through 5. Otherwise stated, all the samples for CD and UV-melting experiments were prepared in buffer containing 10 mM sodium phosphate, 0.1 mM EDTA, 100 mM NaCl at pH = 7.4. The concentration of oligomer was 5 μM strand each. The CD spectra were recorded at 25°C. Inset: UV-Vis spectra of PNA1 through 5 at 90°C, demonstrating that they had the same concentration. T: modified monomer with the indicated side-chain.
