Table 2.
T m s (°C) of PNA-DNA hybrid duplexes containing perfectly matched and single-base mismatched sequences.
| Oligomer | X = A | T (ΔT m) | G(ΔT m) | C(ΔT m) |
|---|---|---|---|---|
| PNA1 (Unmod.) | 47 | 35 (−12) | 32 (−15) | 31 (−16) |
| PNA2 (Ala) | 51 | 40 (−11) | 34 (−17) | 33 (−18) |
| PNA3 (Val) | 51 | 40 (−11) | 34 (−17) | 33 (−18) |
| PNA4 (Ile) | 51 | 39 (−12) | 34 (−17) | 33 (−18) |
| PNA5 (Phe) | 51 | 40 (−11) | 35 (−16) | 35 (−16) |
| PNA6 (Phe 3alt) | 58 | 45 (−13) | 42 (−16) | 39 (−19) |
| PNA7 (Phe 3con) | 60 | 47 (−13) | 41 (−19) | 41 (−19) |
| PNA8 (Val 3alt) | 55 | 41 (−14) | 37 (−18) | 38 (−17) |
| PNA9 (Val 3con) | 55 | 42 (−13) | 37 (−18) | 39 (−16) |