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. Author manuscript; available in PMC: 2011 Jul 18.
Published in final edited form as: Immunol Rev. 2009 Nov;232(1):84–98. doi: 10.1111/j.1600-065X.2009.00840.x

Fig. 1. SLP–YFP internalizes in uncoated structures.

Fig. 1

Jurkat T cells expressing SLP–YFP were plated onto stimulatory coverslips, fixed after incubation at 37 °C for 5 min, stained with anti-GFP antibodies, and processed for electron microscopy. Enhanced gold staining is seen on uncoated pits, vesicles, and internal membranes. Arrowheads indicate pits and vesicles; Arrows indicate tubules, e-endosomes, G-Golgi, c-cap shaped structures similar to recycling endosomes. Insert shows higher magnification view of pits budding from the plasma membrane. Bar (main panel) = 280 nm, bar (insert) = 170 nm. Originally published in Traffic 2006;7:1143–1162, Publisher Wiley-Blackwell.