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FGF21 acutely induces FoxO1 target gene expression in the liver. FGF21 significantly increases IRS2 (panel A) and IGFBP1 (panel B) mRNA expression in the liver. mRNA expression of 3-hydroxybutyrate dehydrogenase type 1 (BDH1) (panel C) and CPT1a (panel D) are also shown. Data are shown as mean ± sem (*, P < 0.05; **, P < 0.00; ***, P < 0.000).
