FIGURE 2.

Effect of elevated glucose concentrations on the expression of the genes implicated in Zn²⁺ homeostasis in mouse pancreatic islets. Mouse islets were incubated for 1 h at 3 mm glucose before incubation for 24 h (A) or 2 h (B) with either 3 mm (white bars) or 16.7 (black bars) glucose. Total RNA was extracted, and qPCR analysis of Slc39a1–12, Slc30a1–10, Mt-1, and Mt-2 was performed. The mRNA levels were normalized to those of a housekeeping gene (cyclophilin A). Bars represent mean ± S.E. (n = 4). *, p < 0.05.