TABLE 1.
Crystallographic studies on SapSLH
| Data Collection | |
| Space group | P41212 |
| Unit cell (Å) | a = b = 77.77, c = 100.41 |
| MW Da (residue) | 24353.4 (223)1 |
| Mol (AU) | 1 |
| SeMet (AU) | 6 |
| Wavelength(Å) | 0.9794 (peak) |
| Resolution range (Å) | 54.96–1.80 (1.80–1.85)2 |
| Number of unique reflections | 27750 (2003) |
| Redundancy | 11.1 (6.3)2 |
| Completeness (%) | 99.86 (98.6) |
| Rmerge (%) | 0.099 (0.748)2 |
| I/ σ(I) | 26.83 (1.61)2 |
| Phasing | |
| RCullis (anomalous) (%) | 0.70 |
| Figure of merit (%) | 0.2498 |
| Refinement | |
| Resolution | 54.96–1.80 |
| Reflections (work/test) | 27750/1486 |
| Rcrystal/Rfree (%) | 16.6/18.9 |
| Rms deviation from ideal geometry | 0.017/1.485 |
| Bond length (Å)/angle (°) | |
| Number of protein atoms | 1741 |
| Number of sulfate/water molecules | 1/241 |
| Mean B-value (Å2) (mainchain/sidechain) | 1.399/3.177 |
| Ramachandran plot statistic (%) | |
| Residues in most favored regions | 92.0 |
| Residues in additional allowed regions | 8.0 |
1 Includes His tag.
2 Numbers in parenthesis include highest resolution shell.