FIGURE 7.

Quantitative PCR of matrix gene expression in unwounded and wounded skin from WT, ts5^−/−, ts5^−/−/Cd44^−/−, and Cd44^−/− mice. RNA preparation and PCRs for aggrecan (agg), versican (vers) V1, col1a, and col3a transcript abundance were performed as described under “Experimental Procedures.” Data for wounded tissue (at day 4 = granulation tissue; day 8 = contraction, and day 15 = dermal regeneration) are expressed as mRNA abundance (arbitrary units) relative to the unwounded (UW) wild type value that was set at unity. Each data point represents the mean ± S.D. of triplicate assays from three individual mice. See also supplemental Table 1 for basal level expression of all genes in all mice lines. Asterisk indicates the restoration of gene expression to WT values.