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NCAM silencing promotes the differentiation of B16F0 cells. A, morphology of siCTRL and siNCAM cells is shown. Scale bar, 25 μm. Expression of E-cadherin was determined by immunoblotting (B) and by real-time PCR (C). *, p < 0.05. D, pigmentation was seen in cells cultured for 3 days. E, quantitative analysis of pigment in medium is shown. *, p < 0.05; n = 3. F, mRNA level of tyrosinase by real-time PCR is shown. *, p < 0.05.
