FIGURE 4.

β-Catenin is involved in the reduced proliferation of B16F0 cells. A, immunoblotting shows the reduced expression level of β-catenin and phosphorylation of GSK-3β at Ser-9 as well as the elevated phosphorylation of β-catenin at Ser 33/37 in cells transfected with NCAM siRNA. β-Actin was used as loading control. B, shown is expression of cytosol β-catenin and nuclear Cyclin D1 by immunoblotting. GAPDH and Lamin B were used as loading control respectively. C, the expression levels of Axin2 and MITF by real-time PCR are shown. *, p < 0.05. D, expression of β-catenin in cells transiently transfected with β-catenin siRNA and their proliferation are shown. *, p < 0.05. E, cell proliferation was examined in siNCAM cells overexpressing wild type of β-catenin or the empty vehicle. *, p < 0.05. Representative pictures of three independent experiments are shown.