FIGURE 5.

NCAM-silencing reduced β-catenin signaling is independent of canonical Wnt and PI3K-Akt pathways. A, immunoblotting showed the phosphorylation of LRP5/6 in cells. β-Actin was used as loading control. B, mRNA levels of Wnt3A and its receptors Frizzle7, Frizzle8, LRP5, and LRP6 were determined by real-time PCR. C, immunoblotting showed the levels of p-Akt and p-ERK in cells. D, the Myc-tagged DN-Akt and vehicle were transiently transfected into B16F0 cells followed by detection of Myc, p-CREB, p-GSK-3β, and β-catenin by immunoblotting. E, B16F0 cells were treated with AKTI (AKT inhibitor) at different concentrations for 12 h. The levels of p-AKT, p-GSK-3β, and β-catenin were determined by immunoblotting. Representative pictures of three independent experiments are shown.