NCAM is able to interact with FGFR, GSK-3β, and β-catenin. A, cell lysates were immunoprecipitated (IP) with anti-NCAM antibody or unrelated isotype IgG and analyzed for the presence of NCAM and β-catenin by Western blot (IB). B, cell lysates were immunoprecipitated with anti-NCAM antibody or IgG and analyzed for NCAM and GSK-3β. C, cell lysates were immunoprecipitated with anti-FGFR antibody or IgG and analyzed for the presence of FGFR, NCAM, β-catenin and GSK-3β. D, immunocytochemistry results indicated the co-localization (yellow, arrows) of NCAM (red) with β-catenin (upper panel, green) and GSK-3β (lower panel, green). Scale bars, 20 μm. Representative pictures of three independent experiments are shown.