FIGURE 2.
Effects of chloroquine and ammonium chloride on D′D3 dimerization. BHK-fur4-D1D2D′D3-FLAGNT-c-MycCT cells (A and B) or BHK-fur4-D1D2-KSDR-D′D3-FLAGNT-c-MycCT cells (C) were treated with the indicated concentration of chloroquine (A) for 48 h or NH4Cl for 72 h (B and C). Conditioned medium was analyzed by SDS-PAGE (4–15%) and Western blotting with HRP-conjugated rabbit anti-human VWF antibody, which preferentially detects products containing D′D3 domains. The VWF propeptide, domains D1D2, can be seen as a weakly reactive 95-kDa band in some lanes. The mass in kDa of marker proteins is indicated at the left.