FIGURE 1.

TRIM32 sensitizes cells to TNFα-induced apoptosis. HEK293T cells stably transfected with a TRIM32-expressing plasmid or control vector were selected in the presence of 1 μg/ml puromycin. A, cell lysates were analyzed by immunoblotting with an affinity-purified anti-TRIM32 antibody. β-Actin was assessed as a loading control. B, cells were treated with 50 ng/ml TNFα for 6 h, stained with annexin V-FITC, and observed under a fluorescence microscope. C, caspase-3 activity was detected with a fluorometric assay, using DEVD-AMC as a substrate. Data are expressed as the mean ± S.D. of three independent experiments.