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. 2011 Apr 25;286(29):25823–25834. doi: 10.1074/jbc.M110.198267

FIGURE 1.

FIGURE 1.

Effects of FGF12 on radiation-induced apoptosis in intestinal crypt cells. BALB/c mice were given TBI at 12 Gy to induce apoptosis in the crypts of the jejunum. Recombinant FGF12B (R&D) was administered intraperitoneally without heparin to BALB/c mice at the indicated doses 24 h before TBI. A TUNEL assay and staining with anti-cleaved caspase 3 antibody were performed on paraffin-embedded sections to evaluate apoptosis in the crypts of the jejunum, as described previously (4). A, jejunum was removed from treated mice 24 h after irradiation. The number of TUNEL+ cells was determined in each crypt by screening more than 10 crypts. B, number of cleaved caspase 3-positive cells was determined in each crypt 8 h after irradiation. All values are means ± S.D. (n = 6). Similar findings were observed in two independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001.