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Analysis of the product formed by WT and HPPD mutants. Representative HPLCs illustrating the product formed by WT and HPPD mutants. HPLC was run on an ODS2 C18 with an isocratic mobile phase containing H2O/acetonitrile/acetic acid (90–10-0.1) at a flow rate of 1 ml/min. Absorbance was recorded at 230 nm. C1-hydroxylated intermediate corresponds to the alternative product released by S246A and N261D HPPD mutants.
