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Rtt107 interacted with the SMC5/6 complex via the Nse6 subunit in a yeast two-hybrid analysis. Rtt107 was fused to the Gal4 DNA binding domain (GBD), and the individual SMC5/6 subunits were fused to the Gal4 activation domain (GAD). Physical interaction was indicated by growth of the yeast on SC-His and/or SC-Ade selection media. The N-terminal and C-terminal fragments of Rtt107 used here were equivalent to the constructs in Fig. 1D.
