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. 2011 May 26;286(29):25903–25921. doi: 10.1074/jbc.M111.243030

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — GRAF1 expression is induced in differentiating myoblasts. A, Western blot analysis of GRAF1 protein levels in embryonic (E), postnatal (P), and adult (Ad) rat hind-limb muscle or brain at the indicated time of development. B, C2C12 cells maintained in growth (G) media or exposed to differentiation conditions (LSM) for the indicated times were lysed, and cell extracts were immunoblotted with GRAF1 and tubulin-specific antibodies. C, C2C12 cells were treated as described in B, and lysates were subjected to an ELISA-based RhoA activity assay. Data are expressed as average relative light units (RLU) per time point (n = 3).