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. 2011 May 26;286(29):25903–25921. doi: 10.1074/jbc.M111.243030

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 10. — GRAF1 is essential for maintaining somite architecture and myoseptal boundaries. A, Western blot analysis for GRAF1 in Con MO- and GRAF1 Mo-injected embryos at the indicated stages of development. IB, immunoblot; St., stain. B, stage 39 GRAF1 Mo-injected embryos exhibit edema (panel b) and anteroposterior axis defects (panel c) relative to control embryo (panel a). C, laser scanning confocal images of whole-mount stage 30–37 Con Mo- and GRAF1 Mo-injected somites reveal progressive degeneration. Embryos were stained with Tm or laminin (LAM) to visualize cells or myosepta, respectively.