FIGURE 1.

Schematic outline of a typical workflow for tissue samples. Sample pretreatment steps include cutting and mounting the tissue section on a conductive target as well as paraffin removal and antigen retrieval for FFPE sections. A frozen serial section or the FFPE section to be analyzed is stained, a digital image is taken, and areas of interest are selected by a pathologist. Trypsin and/or matrix is applied to the tissue section, and mass spectra are generated at each x,y coordinate. IHC, immunohistochemistry.