TABLE 1.

ClpB variants used in this work and their properties

Variant	NBD1	NBD2	Mutation
WT	Binding and hydrolysis	Binding and hydrolysis	None
T1T2	Binding	Binding	E276A/E678A
T1N2	Binding	None	E276A/K611A
N1T2	None	Binding	K212A/E678A
N1N2	None	None	K212A/K611A
T1	Binding	Binding and hydrolysis	E276A
T2	Binding and hydrolysis	Binding	E678A