TABLE 2.

Isothermal titration calorimetry analysis of nucleotide binding to WT ClpB and different protein variants

Experiments were repeated at least two times using two different protein batches. −, interaction was not detected.

ClpB	n	kd	ΔH	−TΔS	ΔG
		μm	kcal/mol	kcal/mol	kcal/mol
ADP
wt	0.8 ± 0.1	5.8 ± 1.7	−10.8 ± 1.2	3.6 ± 1.0	−7.2 ± 0.2
ATP γS
wt	0.9 ± 0.2	3.8 ± 2.6	−11.5 ± 3.1	4.1 ± 4.0	−7.4 ± 0.3
T1T2	0.7 ± 0.1	3.1 ± 0.2	−13.1 ± 0.2	5.6 ± 0.1	−7.5 ± 0.1
ATP
T1T2	1.0 ± 0.1	3.1 ± 0.3	−4.9 ± 0.06	−2.6 ± 0.1	−7.5 ± 0.1
T1N2
N1T2	0.8 ± 0.1	6.9 ± 2.0	−4.2 ± 1.1	−2.8 ± 0.9	−7.0 ± 0.2