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Estimation of the affinity for nucleotides of the NBD1 of WT ClpB and selected protein variants by partial proteolysis
Experiments were repeated at least three times.
| Nucleotide | ClpB | Kd | nH |
|---|---|---|---|
| μm | |||
| ATP γS | wt | 50 ± 6 | 1.0 ± 0.2 |
| ATP | T1T2 | 61 ± 5 | 0.9 ± 0.1 |
| T1N2 | 163 ± 9 | 1.1 ± 0.1 | |
| ADP | wt | 35 ± 4 | 1.2 ± 0.2 |
| T1T2 | 43 ± 4 | 0.9 ± 0.1 | |
| T1N2 | 39 ± 3 | 0.9 ± 0.1 |
