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Artificial oligomerization of the CARD of RIG-I results in signaling to activate the IFN-β and IL-6 genes. A, schematic representation of the FK/RIG fusion protein and its oligomerization by a cross-linking reagent, AP20187. B, HeLa cells stably expressing FK/RIG were treated with AP for the period indicated and cells were harvested to examine endogenous IFN-β and IL-6 mRNA levels by real-time PCR.
