TABLE 2.
Peptide release rates of rRNA mutants
S.E. are calculated from at least three independent determinations.
| Codon (RF) | WT (krel) | A1913U (krel) | G1922A (krel) | % Codona |
|---|---|---|---|---|
| s−1b | s−1b | s−1b | ||
| UAA (RF1) | 0.92 (0.21) | 1.1 (0.20) | 1.2 (0.16) | 0.21 |
| UAG (RF1) | 0.89 (0.23) | 0.87 (0.19) | 0.85 (0.22) | 0.03 |
| UAA (RF2) | 2.1 (0.46) | 2.0 (0.28) | 1.4 (0.35) | 0.21 |
| UGA (RF2) | 1.0 (0.10) | 0.92 (0.19) | 0.08 (0.05) | 0.09 |
a % Codon values calculated for E. coli K12 from the Genomic tRNA Database.
b krel is calculated at 0.25 μm ribosomes and 5 μm release factor.