Table 1.
Confocal FRET analysis of D1 and D2 receptor interaction
| ROI | Donor of FRET | Acceptor of FRET | PFRET | FRET Efficiency |
Distance between donor and acceptor (nm) |
|---|---|---|---|---|---|
| (1) Donor alone | 13.944 | 0 | 0 | 0 | 10 |
| 2 | 842.685 | 562.542 | 529.703 | 0.357 | 5.91 |
| 3 | 804.879 | 488.573 | 474.042 | 0.351 | 5.9 |
| 4 | 830.377 | 569.241 | 535.203 | 0.353 | 5.924 |
| 5 | 720.099 | 436.039 | 410.781 | 0.319 | 6.269 |
| 6 | 898.475 | 482.132 | 444.885 | 0.311 | 6.171 |
| 7 | 964.916 | 460.029 | 407.186 | 0.247 | 6.875 |
| 8 | 1116.854 | 399.85 | 384.365 | 0.234 | 6.632 |
| 9 | 951.224 | 324.177 | 314.284 | 0.206 | 7.145 |
| 10 | 1076.73 | 341.095 | 326.925 | 0.2 | 7.153 |
| 11 | 976.861 | 227.299 | 216.367 | 0.149 | 7.789 |
| 12 | 1201.314 | 363.612 | 336.45 | 0.191 | 7.121 |
| 13 | 998.373 | 283.121 | 269.621 | 0.187 | 7.197 |
| 14 | 1017.225 | 303.213 | 287.876 | 0.2 | 6.987 |
| 15 | 816.347 | 166.339 | 156.562 | 0.129 | 8.069 |
| 16 | 806.034 | 265.133 | 251.731 | 0.19 | 7.393 |
| 17 | 815.063 | 349.81 | 338.709 | 0.252 | 6.792 |
| 18 | 833.344 | 485.752 | 382.262 | 0.257 | 6.946 |
| (19)Non-Specific | 95.52 | 83.573 | 35.284 | 0.086 | 9.168 |
| Average | 921.8117 | 382.821 | 356.88 | 0.243117 | 6.83958 |
| SEM | 33.82434 | 29.9949 | 27.1577 | 0.018620 | 0.165392 |
Confocal FRET analysis of figure 1 shows the relative expression of the donor (D2-Alexa 350, green) and acceptor (D1-Alexa 488, red). The analysis also shows the processed FRET (pFRET), the FRET efficiency and the distances separating the two fluorophore-tagged receptors in each microdomain (ROI), with averages and SEM in the bottom of the table. A distance ~10 nm or higher indicates no FRET.