Fig. 1.

Conditional inactivation of SUR-8 in endothelial cells. A: Schematic illustration of mouse Sur-8 wild-type allele, targeting vector and the loxP modified Sur-8 loci. Breeding the mice containing the targeted allele with Actin-Flpe transgenic mice results in pups with the flox allele. The disrupted (Δ) allele was then obtained by crossing the flox mice with PGK-Cre mice. Restriction enzyme sites: X, XbaI; B, BglII; and S, SpeI. B: RT-PCR analysis of isolated endothelial cells using primers specific to Sur-8 gene. GAPDH was used as an internal control. C: Western blotting analysis with an anti-SUR-8 antibody on isolated endothelial cells derived from different genotypes. GAPDH was used as an internal control. wt, Sur-8^+/flox; cko, Sur-8^Δ/flox;Tie2-Cre; ctr, Sur-8^Δ/flox.