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Nested polymerase chain reaction of EBA-175 products. Lane 1: 200 bp molecular ladder; Lane 2: negative control; Lane 3: PCR positive control (C-fragment); Lane 4: C-fragment of 714 bp; Lane 5: F-fragment of 795 bp; Lane 6: Mixed infection (F- and C-fragment); Lane 7: 200 bp molecular ladder.
