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. 2011 Jun 27;8:324. doi: 10.1186/1743-422X-8-324

Figure 2.

Figure 2

Interaction of Apoptin with Gallus Hsc70 in DF-1 cells. (A) Interaction of Apoptin with exogenous Hsc70 in DF-1 cells. DF-1 cells were co-transfected with plasmids encoding Myc-tagged Apoptin, Flag-tagged Hsc70, or empty plasmid (pCMV-Myc or pRK5F vector as a control) in the indicated combinations. Twenty-four hours after transfection, cell lysates were immunoprecipitated with anti-Myc monoclonal antibody and were analyzed by Western blot using anti-Myc or anti-Flag antibodies. (B) Interaction of Apoptin with endogenous Hsc70 in DF-1 cells. DF-1 cells were transfected with plasmid encoding Myc-tagged Apoptin or empty plasmid (pCMV-Myc vector as a control). Twenty-four hours after transfection, cell lysates were immunoprecipitated with anti-Myc monoclonal antibody and were analyzed by Western blot using anti-Myc or anti-Hsc70 antibodies.