Figure 4.

Determination of Gallus Hsc70-binding domain for Apoptin. (A) Schematic representation of the genes encoding full length and truncated Apoptin molecules (Δ1 through 3). The numbers indicate amino acids positions within the proteins and known functional regions are indicated. (B) Apoptin interacts with various truncated Hsc70 molecules. DF-1 cells were co-transfected with pCMV-Myc-Apoptin, full length pEGFP-Hsc70, truncated pEGFP-Hsc70 (Δ1: 386-646aa; Δ2:386-544aa; Δ3: 545-646aa) or empty pEGFP vector as a control. Twenty-four hours after transfection, cell lysates were immunoprecipitated with anti-Myc monoclonal antibody and were analyzed by Western blot using anti-Myc or anti-GFP antibodies.