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X-band EPR spectra of (CuII+αKG)FIH variants. FIH and mutants (1 mM), CuSO4 (0.9 mM), αKG (1 mM) in 50 mM HEPES pH 7.50. 9.438 GHz frequency, 20 mW power, 20G modulation amplitude, 100 GHz modulation frequency, 327 ms time constant at 77 K.
