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Coupling and yield of hydroxylated CTAD by FIH variants.
| Ca | % CTADOHb | |
|---|---|---|
| WT-FIH | 0.98 ± 0.03 | 94% |
| N205A | 1.08 ± 0.04 | 90 |
| N294A | 1.3 ± 0.1 | 79 |
| Q239N | 3.1 ± 0.4 | 23 |
| R238M | n.d. | 5 |
C = [succinate]/[CTADOH] as determined under steady-state turnover conditions; see text for details. Not determined (n.d.).
Qualitative endpoints: FIH (100 μM) FeSO4 (500 μM), αKG (500 μM), CTAD (50 μM) 50 mM HEPES, pH 7.50.
