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. Author manuscript; available in PMC: 2011 Jul 18.
Published in final edited form as: Vaccine. 2009 Dec 23;28(7):1746–1757. doi: 10.1016/j.vaccine.2009.12.016

Fig. 7.

Fig. 7

Immunological studies from BALB/c mice that were vaccinated against parasite antigen (LsAg) and repeatedly injected with LsAg or PBS for two (○) or eight weeks (●). (A) LsAg-specific and total IgE levels after 2 and 8 weeks of repeated LsAg/PBS injections. (B) Frequency of IL-4 positive basophils in response to LsAg after 2 or 8 weeks of repeated injections. (C) Splenic IL-4, IL-5, and IFNγ production in response to LsAg and (D) anti-CD3/anti-CD28 after eight weeks of repeated exposures to LsAg or PBS. (E) Spleen cell proliferation in response to LsAg or anti-CD3/anti-CD28 after eight weeks of repeated injections. (F) Spontaneous frequency of CD4+CD25+FoxP3+ splenic T cells after eight weeks of repeated exposures. (G) Splenic IL-10 production in response to LsAg and anti-CD3/anti-CD28 from eight week repeatedly injected mice. Timepoints listed are duration of RPEs. Shown are medians. Significant differences were analyzed by Mann-Whitney-U-test (*p<0.05, **p<0.01, ***p<0.001).