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. Author manuscript; available in PMC: 2012 Jul 7.
Published in final edited form as: Lab Chip. 2011 May 20;11(13):2212–2221. doi: 10.1039/c1lc20111e

Figure 6.

Figure 6

Screening for cryoprotection of DMSO-PBS mixtures. The cell suspension droplets were mixed with DMSO-PBS mixtures and then frozen/thawed. After F/T, these droplets were further mixed with the viability dye droplets to assess post-thaw viability. (a) Fluorescence micrographs of cell suspension droplets after F/T. (b) Post-thaw cell viability with respect to DMSO concentrations (v/v %). The EWOD results imply that 12.5% and 25% DMSO mixtures are the optimal conditions for cryoprotection of MCF-7 cells. The differences of EWOD and macro experiments are not statistically significant (p > 0.05) for all experimental points (n ≥ 3 for each point).