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. 2011 Jun 22;31(25):9159–9169. doi: 10.1523/JNEUROSCI.0302-11.2011

Figure 6.

Figure 6.

Effects of exogenous expression of NF-κB subunits on eGFP expression, GLT-1 expression, and activation of the GLT-1 promoter reporter. A, B, Cortical astrocytes were transfected (FuGENE) with p50, p65, p50 with p65, or dsRED plus the GLT-1 promoter reporter. After 10 d, the levels of eGFP (A) or GLT-1 (B) were analyzed by Western blot. Fifteen micrograms of cell lysate was loaded in each lane. Bottom, Summary of quantification of eGFP and GLT-1 protein levels normalized to actin and expressed relative to the levels observed in p65, p50, or p65 with p50 transfected astrocytes, respectively. C, Luciferase activity was measured, normalized to protein, and expressed relative to that observed in astrocytes transfected with empty vector. Data are the mean ± SEM of three to five independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 compared with corresponding dsRED transfected control; ###p < 0.001 compared with astrocytes transfected with wild-type promoter fragment and either of the NF-κB subunits.