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. Author manuscript; available in PMC: 2012 Jun 28.
Published in final edited form as: Biochemistry. 2011 Jun 6;50(25):5767–5779. doi: 10.1021/bi2005416

Figure 2.

Figure 2

Rate and product data for the reaction of [1-13C]-GA catalyzed by Tbb TIM in the presence of 15 mM phosphite dianion in D2O buffered by 20 mM imidazole at pD 7.0 and 25 °C (I = 0.1, NaCl), determined by 1H NMR spectroscopy. (A) The decrease with time in the fraction of remaining [1-13C]-GA. (B) The change with time in the fractional yields of the products, fP, of the phosphite-activated TIM-catalyzed reaction of [1-13C]-GA. The initial product yields reported in Table 3 were obtained by making a short linear extrapolation of product yields to zero reaction time. Key: (▼) yield of [2-13C]-GA; (◆) yield of [2-13C, 2-2H]-GA; (■) yield of [1-13C, 2-2H]-GA.