FIGURE 3.

Effect of 2′-deoxy modifications in the template on polymerization efficiency, in the context of truncated P2 oligos. (A) Numbering of 2′-hydroxyl groups relative to the 3′-terminal nucleotide of the primer. (B) Quantitation of 2′-deoxy effects in the presence of the RNA P2 oligos 5′-GGCACCA (filled circles), 5′-GGCACC (filled squares), 5′-GGC (open squares), or in the absence of a P2 oligo (open circles). The graph shows the fold reduction in the rate of primer extension when the 2′-hydroxyl group at a given position in the template was replaced by a 2′-deoxy modification. The difference in the 2′-deoxy effects between P2 oligos 5′-GGCACCA and 5′-GGC shows that the 2′-deoxy effect is caused by the 3′-terminal sequence “ACCA.” Error bars are standard deviations of three independent experiments. Error bars smaller than the symbol are hidden by the symbol.