FIGURE 5.

Effect of the nucleotide composition of 15-nt randomized DNA P2 oligo 3′-extensions on polymerization efficiency. Representative autoradiograms of PAGE-separated polymerization products are shown, for reactions with the four different templates T21, T50a, T50b, and T50c. Only the 3′-extensions to the DNA P2 oligo sequence 5′-GGCGCC are shown. The number of nucleotides added to the primer during polymerization is indicated. Asterisks mark the four P2 oligos that mediated the highest polymerization efficiency, averaged over all templates. The values of polymerization efficiency are given in Supplemental Table S3.