FIGURE 7.

Effects of P2 oligo extensions on polymerization efficiency. Autoradiograms of PAGE-separated polymerization products are shown, for reactions with the template T50c. (A) Comparison between three separate reactions with three different P2 oligos containing the 3′-extensions ext1, ext2, and ext3, and a reaction containing the same three P2 oligos in one reaction mixture. The number of nucleotides (nts) that were added to the primer is indicated. The polymerization efficiencies are given in the text, with errors as standard deviations from triplicate experiments. (B) Comparison of four ribozyme/P2 oligo contexts. The polymerase ribozyme Pol1e with the wild-type RNA P2 oligo 5′-GGCACCA is compared to ribozyme B6.61 without P2 oligo, polymerase ribozyme Pol1e with the truncated DNA oligo 5′-GGCGCC, and polymerase ribozyme Pol1e with DNA P2 oligo 5′-GGCGCC-ext3. The polymerization reaction times are indicated.