FIGURE 8.

Influence of P2 oligo extensions on polymerization fidelity. The fidelity was calculated as the percent incorporation of the templated nucleotide. Four templates were used, T21, T50a, T50b, and T50c. The symbols denote the wild-type RNA P2 oligo (white circles), the DNA P2 oligo with extension (N)₁₅ (gray triangles), and the DNA P2 oligo with the most efficient (G/T)₁₅ extension (ext3; black squares). Missing symbols indicate that the values could not be obtained due to low polymerization efficiency with the specific P2 oligo/template combinations. Errors are standard deviations from triplicate experiments, and are smaller than the symbol when they are not visible.