Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
γ-toxin cleavage requires a ribose 5′ of the scissile wobble nucleotide. The 17-mer oligonucleotide substrates are shown. The dC-6, dU-7, dA-11, and dC-12 substrates contain single deoxynucleosides in the anticodon loop, as indicated. Nuclease reaction mixtures (10 μL) contained 200 fmol 5′ 32P-labeled 17-mer substrates and γ-toxin as specified. The extents of 17-mer cleavage are plotted as a function of input γ-toxin.
