Table 1.
Antibodies Used Against Markers of Epithelial Function, Stromal-Epithelial Interaction, and Angiogenesis
| Type of Marker | Dilution | Supplier |
|---|---|---|
| Intracellular signaling pathways | ||
| ki67 | 1:50 | Dako North America, Carpinteria, CA |
| bcl2 | 1:200 | Dako North America |
| p53 | 1:1000 | Dako North America |
| Chromogranin | 1:200 | Dako North America |
| Synaptophysin | 1:25 | Dako North America |
| IGF-I | 1:200 | Abcam, Cambridge, MA |
| IGF-IRB | 1:50 | Cell Signaling Technology, Danvers, MA |
| Stromal-epithelial interaction pathways | ||
| AR | 1:50 | Dako North America |
| pAR | 1:20 | Imgenex Corp, San Diego, CA |
| CYP17 | 1:100 | Novus Biologicals, Littleton, CO |
| SRD5A1 | 1:400 | Novus Biologicals |
| Sonic hedgehog | 1:100 | Santa Cruz Biotechnology, Santa Cruz, CA |
| Ptch (patched) | 1:350 | Strategic Diagnostics, Newark, DE |
| Smo (smoothened) | 1:80 | Abcam |
| Gli1 | 1:300 | Novus |
| Gli2 | 1:600 | Abcam |
| Angiogenesis | ||
| VEGF | prediluted | Abcam |
| CD31 | 1:30 | Dako North America |
Abbreviations: IGF-I, insulin-like growth factor I; IGF-IRB, IGF-I receptor B; AR, androgen receptor; pAR, phosphorylated androgen receptor; VEGF, vascular endothelial growth factor.