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EB3 localizes to the tip of motile cilia of bronchial epithelial cells. Human bronchial epithelial cells were fixed with cold methanol followed by 4% formaldehyde in PBS. The cells were stained with antibodies against EB3 (green) and acetylated α-tubulin (red) to label cilia; nuclei were visualized with DAPI (blue). The image shows a side view of a single cell. Note the presence of EB3 at the tip as well as the base of cilia.
