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. 2011 Aug 1;124(15):2561–2572. doi: 10.1242/jcs.085001

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Fig. 12. — FoxL2 binds to and upregulates mouse Acta2 promoter activity. (A) FoxL2 binds to mActa2 promoter in ChIP assay. Predicted FoxL2 binding sites within the mActa2 5′-flanking regulatory region (~1.1 kb). Arrows indicate DNA primers used in ChIP assay for FoxL2 binding site. A 235 bp PCR-amplified product was generated from ChIP with anti-Myc tag antibody (lane 3) but not from mouse IgG (lane 4). (B) FoxL2 enhances mActa2 promoter luciferase activity in vitro. Diagram of mActa2 promoter-luciferase construct. Co-transfection of pcDNA3.1-mFoxL2 plasmid enhances luciferase activity threefold.